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ARS ON-LINE SAMPLING SYSTEMS

*Fermentation Production - Online HPLC Analysis of Fermentors Acrobat PDF
In this application note Groton Biosystems describes the use of the ARS-M™ Automated Reactor Sampling System for online HPLC for rapid analysis of fermentation tanks. In this example, bacterial fermentation is converting sugars to organic acids. The interface to the fermentor employs an in-situ membrane probe to separate the solid and liquid components of the fermentation broth. This filtrate is then delivered directly through an automatic injection loop (Shimadzu Prominence HPLC). The HPLC data with OPC feedback control, can be used to adjust the feed, optimize the growth of biomass, and improve the yield of the desired fermentation product.

*Integrated Reactor Feeding System - Amino Acid Feedback Control and Productivity Gains - E.Coli Acrobat PDF
A study was conducted to demonstrate the efficiency and viability of a fully automatic reactor feeding system to regulate essential amino acid concentrations in a microbial fermentation. These studies were conducted using an online amino acid HPLC system integrated with an ARS-M Reactor Sampling System and a programmable Reactor Control System controlling amino acid feedstock pumps. Baseline studies were conducted to determine which amino acids were consumed by the fermentation process and the rates of consumption. Subsequent studies were conducted in which those amino acids were fed to maintain a desired concentration for the entire course of the fermentation. Green Fluorescent Protein ("GFP") was used as a marker for specific productivity measurement. Initial results indicate that productivity is positively correlated to maintaining appropriate levels of essential amino acids. Future studies are in progress to optimize the media and feedstocks.

Biogen IDEC - On-line Amino Acid Analysis from a Small Scale CHO Cell Bioreactor Acrobat PDF
This Application Note describes a fast, accurate and reproducible measurement of 21 amino acids in a Chinese Hamster Ovary (CHO) cell culture using an online HPLC instrument. The hyphenated system combines the automatic sampling and reactor sterility protection of the Groton ARS with a high performance amino acid separation system provided by the Agilent 1100 Series HPLC System. The set-up, which is described in this Application Note, was used for in-process analysis during the course of a 14-day bioreactor run, which has been previously demonstrated using pulsed amperomeric detection. This tool provides immediate sampling and testing of a bioreactor that does not compromise bioreactor or samples, reduces hands on work and improves in-process monitoring of a bioreactor.

DASGIP/YSI/Groton - Automated On-Line Real Time Reactor Feedback Control Acrobat PDF
Integration of the Groton Automated Reactor Sampler, the DASGIP Bioreactor and the YSI Biochemistry Analyzer - Bioprocess monitoring and control of key analytes such as glucose, lactate, amino acids, and ammonia levels is critical to obtain optimal and consistently high productivity of a cell culture system. Improved productivity is critical to the development of high efficiency, economical production processes for therapeutic proteins and monoclonal antibodies. Despite the apparent value of feedback control systems in the biopharmaceutical industry there are few successful demonstrations. Here we present a complete, closed loop feedback control system by integrating a Groton Biosystems Automated Reactor Sample System with an YSI biochemistry analyzer and a DASGIP bioreactor control system in order to achieve a powerful, automated online, real time feedback control system for mammalian cell culture. With real time sampling and feedback control we provide an effective and efficient tool for the optimization of mammalian cell culture bioprocess. This system can easily be adapted to other types of manufacturing systems for biologics, for example, microbial fermentation. In addition, this system would also promote the FDA Process Analytical Technology (PAT) initiative.

DeltaV Systems Integrate Seamlessly Acrobat PDF
Solutions designed for seamless integration with an array of bioprocess control platforms. Integration of these platforms to the ARS can deliver improved process quality and reliability while reducing operating costs. This note focuses on the integration of the ARS to the industry leading DeltaV Bioprocess Controller and the realized benefits from this integration.

Genentech - Implementing an Automated Reactor Sampling System for Monitoring Cell Culture Bioreactors Acrobat PDF
Process Development Engineering. Monitoring of cell culture bioreactors is critical to process control and optimization. Currently offline sampling is used in various cell culture operations. This process is time consuming and labor intensive. Automating the sampling process is highly desired but concerns about sterility, sample volume and cross contamination are common. This paper presents the evaluation of an automated sampling system by Groton Biosystems.

MIT - Sterility Testing Acrobat PDF
Simulating a Bacterial Contamination in a Novel Automated Bioprocess

Online HPLC for Biotechnology: ARS and Agilent 1200 Acrobat PDF
Online analytical instrumentation facilitates productive, efficient monitoring of fermentation or cell culture processes in real time. The ARS in combination with the Agilent 1200 Series LC system introduces and complies with the goals of the FDA's Process Analytical Technology (PAT) initiative. Described here is a new technology which introduces automatic, hands-free, online sampling of HPLC analysis. This Technical Note demonstrates this instrumentation for amino acid analysis.

Pfizer - Performance Evaluation of an Automated Bioreactor Sampling System for Mammalian Cell Cultivation Acrobat PDF
Two common goals of biotherapeutics development are reduction in timelines and increased process understanding. An automated bioreactor sampling system developed by Groton Biosystems has proved its capacity to address those two seemingly conflicting needs for the industry. Paper presented at the ACS Meeting in August, 2008.

U of Maine - On-line Solutions for Bioethanol Fermentation Analyses Acrobat PDF
Yeast fermentation converts sugar to alcohol under anaerobic conditions. In this study and ARS400 was operated to take samples from a BioFlo110 three liter fermentor drawing samples containint E. coli K011 bacteria cells.

Analysis of Native Proteins in Free Capillary Zone Electrophoresis Acrobat PDF

Analysis of Sulfonic Acids in Waste Water Acrobat PDF

Baby Formula Analysis by CE Acrobat PDF

Carbohydrates in Chocolate Extract by CE Acrobat PDF

CE for Food and Beverage Application Acrobat PDF
A fast, verstile, automated solution for analysis of organic and inorganic ions.

Comparison of Anions in a Regular and Diet Cola using Conductivity Detection Acrobat PDF

Determination of Ammonium Content in Saliva Using Conductivity Detection Acrobat PDF

Determination of Organic Acids in Red Wine Acrobat PDF

Diet Cola Analysis by CE Acrobat PDF

Impurities in Insulin by CE Acrobat PDF

Peak Purity Determination using Diode Array Detection Acrobat PDF

Trace Chloride and Sulfate in Glacial Acetic Acid using Conductivity Detection Acrobat PDF