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HPLC Thrives on Speed and Efficiency
Economic Crisis and Demand for Near-Process Analytics Drive Methods Development
Genetic Engineering News
May 01, 2009
George Barringer was recently quoted in Genetic Engineering News.
According to George Barringer, Ph.D., CSO of Groton Biosystems, HPLC/PAT has been slow to catch on because it requires changes "in process and focus on the part of users and suppliers." More complex analyses usually go to an external analytical lab, far from the processing area.
"Moreover practitioners lack the facilities for HPLC, and using it online will require a mindset change." The other hurdle, Dr. Barringer says, has been instrument suppliers, who focus on analytical laboratories to the detriment of process groups.
Yet HPLC is ideally suited for feedback control, particularly for quantifying the appearance of the main product or consumption of amino acids, carbohydrates, and other nutrients. Groton has demonstrated this with its flagship products, in the ARS line of automated auto-samplers and feedback control systems that connect up to eight reactors to four analytical instruments.
The company has collaborated with Agilent (on HPLC systems), DASGIP (bioreactors), and YSI (bioprocess analyzers). Thus far Genentech, Pfizer, Novozymes, and Biogen IDEC have evaluated real-time analysis that combine Groton autosamplers and various analytical instruments.
Groton Biosystems says that its Automated Reactor Sampling System facilitates online HPLC analysis, cell viability, and nutrient monitoring.
To view the entire article go to...
"http://www.genengnews.com/articles/chitem_print.aspx?aid=2895&chid=0"
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