|
Amino Acid Feedback Control-Groton Bioystems has released a study exploring feeding strategies. The study shows that controlled feeding of select amino acids, controlled by the ARS-M, increased production of protein by more than 30%.
March 08, 2010
A study was conducted to demonstrate the efficiency and viability of a fully automatic reactor feeding system to regulate essential amino acid concentrations in a microbial fermentation. These studies were conducted using an online amino acid HPLC system integrated with an ARS-M Reactor Sampling System and a programmable Reactor Control System controlling amino acid feedstock pumps. Baseline studies were conducted to determine which amino acids were consumed by the fermentation process and the rates of consumption. Subsequent studies were conducted in which those amino acids were fed to maintain a desired concentration for the entire course of the fermentation. Green Fluorescent Protein ("GFP") was used as a marker for specific productivity measurement. Initial results indicate that productivity is positively correlated to maintaining appropriate levels of essential amino acids. Future studies are in progress to optimize the media and feedstocks. Click here to download the complete Application Note - http://www.grotonbiosystems.com/company/library_login.cfm?documentID=119
|
