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APPLICATION NOTES

Application Questionnaire (MKT-015) Acrobat PDF
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Biogen IDEC - On-line Amino Acid Analysis from a Small Scale CHO Cell Bioreactor Acrobat PDF
This Application Note describes a fast, accurate and reproducible measurement of 21 amino acids in a Chinese Hamster Ovary (CHO) cell culture using an online HPLC instrument. The hyphenated system combines the automatic sampling and reactor sterility protection of the Groton ARS with a high performance amino acid separation system provided by the Agilent 1100 Series HPLC System. The set-up, which is described in this Application Note, was used for in-process analysis during the course of a 14-day bioreactor run, which has been previously demonstrated using pulsed amperomeric detection. This tool provides immediate sampling and testing of a bioreactor that does not compromise bioreactor or samples, reduces hands on work and improves in-process monitoring of a bioreactor.

DASGIP/YSI/Groton - Automated On-Line Real Time Reactor Feedback Control Acrobat PDF
Integration of the Groton Automated Reactor Sampler, the DASGIP Bioreactor and the YSI Biochemistry Analyzer - Bioprocess monitoring and control of key analytes such as glucose, lactate, amino acids, and ammonia levels is critical to obtain optimal and consistently high productivity of a cell culture system. Improved productivity is critical to the development of high efficiency, economical production processes for therapeutic proteins and monoclonal antibodies. Despite the apparent value of feedback control systems in the biopharmaceutical industry there are few successful demonstrations. Here we present a complete, closed loop feedback control system by integrating a Groton Biosystems Automated Reactor Sample System with an YSI biochemistry analyzer and a DASGIP bioreactor control system in order to achieve a powerful, automated online, real time feedback control system for mammalian cell culture. With real time sampling and feedback control we provide an effective and efficient tool for the optimization of mammalian cell culture bioprocess. This system can easily be adapted to other types of manufacturing systems for biologics, for example, microbial fermentation. In addition, this system would also promote the FDA Process Analytical Technology (PAT) initiative.

Genentech - Implementing an Automated Reactor Sampling System for Monitoring Cell Culture Bioreactors Acrobat PDF
Process Development Engineering. Monitoring of cell culture bioreactors is critical to process control and optimization. Currently offline sampling is used in various cell culture operations. This process is time consuming and labor intensive. Automating the sampling process is highly desired but concerns about sterility, sample volume and cross contamination are common. This paper presents the evaluation of an automated sampling system by Groton Biosystems.

MIT - Sterility Testing Acrobat PDF
Simulating a Bacterial Contamination in a Novel Automated Bioprocess

Pfizer - Performance Evaluation of an Automated Bioreactor Sampling System for Mammalian Cell Cultivation Acrobat PDF
Two common goals of biotherapeutics development are reduction in timelines and increased process understanding. An automated bioreactor sampling system developed by Groton Biosystems has proved its capacity to address those two seemingly conflicting needs for the industry. Paper presented at the ACS Meeting in August, 2008.

U of Maine - On-line Solutions for Bioethanol Fermentation Analyses Acrobat PDF
Yeast fermentation converts sugar to alcohol under anaerobic conditions. In this study and ARS400 was operated to take samples from a BioFlo110 three liter fermentor drawing samples containint E. coli K011 bacteria cells.

ARS Series for Online Ethanol Sampling Acrobat PDF
Groton Biosystems offers high performance instruments to assay the viscous and dense samples found in both corn and biomass fermentation systems. Groton ARS systems provide online analysis by high pressure liquid chromatography (HPLC - sugar and ethanol), fraction collection, and small molecule nutrient monitors.

ARS Series for Online HPLC/Agilent 1200 Series LC System (MKT-032) Acrobat PDF
The ARS system with the Agilent 1200 Series LC provides a high level of automation for development, scale-up, and production within biopharmaceutical facilities.

ARS-M Series Product Bulletin (MKT-092) Acrobat PDF
Automated Reactor Sampling (ARS) Systems deliver precise on-line sampling of bioreactor contents while ensuring complete, sanitary isolation.

Nutrient Monitor - YSI - Interface Acrobat PDF
2700 ARS and 7100 ARS - Automated Online Biochemistry Analyzers

Automated Reactor Sampling and Agilent 1200 Series LC System for Online HPLC Assays Acrobat PDF
Increase efficiency with a powerful process analytical technology solution for the biopharmaceutical Industry.

Simulating a Bacterial Contamination in a Novel Automated Bioprocess Acrobat PDF
Department of Chemical Engineering at Massachusetts Institute of Technology (MIT), Cambridge, MA

ARS - Phase in Closed Loop Solution (MKT-110) Acrobat PDF
Crawl, Walk, Run Approach to Automated Sampling. Start by drawing sample directly to a sample collector, then to analytical instruments and finally to a reactor control system for a complete closed loop solution.

ARS Product List Acrobat PDF
Product listing for automated sampling.

ARS-Interface Steam-in-Place Sterilized Sample Acrobat PDF
Steam Sterilized Sample Interface for Automated Online Sampling from Bioreactors and Fermentors - The Groton Automated Sampling Systems are designed to connect to stock steamable reactor sample valves.

ARS-Interface Sterile Bioreactor Sampling Acrobat PDF
Process which maintains the sterility of a reactor or fermentor during automated sampling.

BioProbe - In-situ Filter Probe Acrobat PDF
Sterilizable, Disposable, Cell Free - Ideal for Cell Culture and Fermentation Applications. Biopharmaceutical, Industrial Microbiology, and Biofuel

Sample Collectors for Automated Reactor Sampling - Refrigerated and Sub-Zero Acrobat PDF
The ARS-M™ Automated Reactor Sampling Systems automatically acquires samples from up to 8 bioreactors and, in our Phase 1 configuration, delivers the sample to a Sample Collector. A dual channel option is available to collect cell containing or cell free samples.

ARS- Closed Loop Solution Acrobat PDF
BioProcess International - Vendor Voice - May, 2008

Automated Bioprocess Sampling and Analysis Acrobat PDF
Genetic Engineering News (GEN)

Automated Sanitary Sampling Acrobat PDF
BioProcess International

Downstream Process Optimization Opportunities Using On-Line and At-Line PAT Instrumentation Acrobat PDF
BioPharm International

Improve Production Efficiencies and Yields Using Automated On-line Sampling Systems for Fermentors and Bioreactors Acrobat PDF
BioProcess International

Increase Efficiency with a powerful Process Analytical Technology Solution for the Biopharmaceutical Industry Acrobat PDF
Agilent - Pharmaceutical Analysis e-Newsletter

Integrated Equipment to Offer 24/7 Sampling and Analysis Solutions for Liquid Processes Acrobat PDF
Technical Insights - Frost & Sullivan

Online Fermentation Analyzer Optimizes Production Acrobat PDF
Ethanol Producer Magazine

Reference Letter - BioTrend Acrobat PDF

Cell Counter Interface to ARS-M Acrobat PDF
Online Automated Sampling for Cell Counting - ARS interfaces to industry standard cell counters and provides a sterile and automatic connection between the bioreactor sample and the cell counter.

DeltaV Systems Integrate Seamlessly Acrobat PDF
Solutions designed for seamless integration with an array of bioprocess control platforms. Integration of these platforms to the ARS can deliver improved process quality and reliability while reducing operating costs. This note focuses on the integration of the ARS to the industry leading DeltaV Bioprocess Controller and the realized benefits from this integration.

Online HPLC for Biotechnology: ARS and Agilent 1200 Acrobat PDF
Online analytical instrumentation facilitates productive, efficient monitoring of fermentation or cell culture processes in real time. The ARS in combination with the Agilent 1200 Series LC system introduces and complies with the goals of the FDA's Process Analytical Technology (PAT) initiative. Described here is a new technology which introduces automatic, hands-free, online sampling of HPLC analysis. This Technical Note demonstrates this instrumentation for amino acid analysis.

Value Proposition for Automated Bioreactor Sampling (MKT-152) Acrobat PDF
Achieve corporate goals and Automate Sampling. Learn how automation will save you money by putting in place the tool to track product quality, increase yield, reduce product development cycles, reduce labor and reduce the human eror factor.

Automated Monitoring Solutions-Video Image (tiff, eps, all other)
Overview of Bioreactor Online Sampling

Financial Calculator Link Text
Interactive calculator demonstrating the savings of automated sampling.